Protein Purification Procedures | Experiment Guru

Protein purification is a process in molecular biology that involves isolating one or a few proteins from a complex mixture, usually cells, tissues, or whole organisms. The purification process typically involves several steps, including cell lysis, clarification, and various separation techniques such as [[chromatography|chromatography]], [[electrophoresis|electrophoresis]], and [[centrifugation|centrifugation]]. These steps exploit differences in protein size, physico-chemical properties, binding affinity, and biological activity to separate the desired protein from other proteins and non-protein components. The process of protein purification has been refined over the years, with the introduction of new techniques and technologies, such as [[affinity-chromatography|affinity chromatography]] and [[gel-electrophoresis|gel electrophoresis]].